Cat.No.
COP33
Species
Streptococcus sp.
ID
193592
Product Name
Recombinant Streptococcal Protein G
Product Overview
Recombinant streptococcal protein G is produced in Escherichia coli using sequence from Streptococcus C1-C2-C3.
Description
Recombinant streptococcal protein G lacking the albumin binding region thereby avoiding undesirable reactions with albumin, though the Fc binding domain is still present.
Source
E. coli
Form
Lyophilized white Powder containing no additives.
Molecular Mass
The Protein G contains amino acids 190-384 having a molecular mass of 21.6 kDa. The Protein-G migrates on SDS-PAGE around 32 kDa.
Purity
>95% as determined by SDS-PAGE and HPLC.
Applications
Protein G binds to the constant region of many species of immunoglobulin G. It can be used to detect, quantify and purify IgG antibodies and antibody/antigen complexes. Recombinant Protein G contains only IgG binding domains. The albumin-binding domain as well as cell wall and cell membrane binding domains have been removed to ensure the maximum specific IgG binding capacity.
Storage
After reconstitution, aliquot and store at -20°C. Avoid repeated freeze/thaw cycles.
Reconstitution
Reconstitution with deionized water or PBS.