Cat.No.
Streptavidin-501
Species
Streptomyces avidinii
Product Name
Recombinant streptavidin
Product Overview
Recombinant streptavidin from Streptomyces avidinii, was produced in Escherichia coli.
Description
Streptavidin is a tetrameric protein composed of identic subunits. Each subunit binds one biotin molecule with a KD of ~1×10^-15M. The preparation contains an N- and C-terminal shortened variant (core streptavidin) with improved properties concerning homogeneity, solubility, resistance towards proteolytic degradation and accessibility of the biotin binding pocket as compared to native streptavidin.
Source
E. coli
Form
Lyophilized in 10 mM potassium phosphate buffer pH 6.5
Molecular Mass
~53,000 per tetramer.
AA Sequence
MAEAGITGTW YNQLGSTFIV TAGADGALTG TYESAVGNAE SRYVLTGRYD SAPATDGSGT ALGWTVAWKN NYRNAHSATT WSGQYVGGAE ARINTQWLLT SGTTEANAWK STLVGHDTFT KVKPSAAS
Endotoxin
Less than 1EU/μg of rStreptavidin as determined by LAL method.
Purity
>95% by SDS-PAGE
Unit Definition
>17 U/mg (one unit binds 1 μg D-biotin at pH 8.9)
Usage
This material is for research, laboratory or further evaluation purposes. NOT FOR HUMAN USE.
Stability
2 years after shipping
Storage
-20 centigrade. Avoid repeated freeze/thaw cycles.
Reconstitution
Dissolve with double distilled water
Shipping
Room temperature
