Cat.No.
srtA-158S
Species
Staphylococcus aureus
Product Name
Recombinant Staphylococcus aureus srtA protein, His-tagged
Product Overview
Recombinant Staphylococcus aureus srtA(26–206 aa) fused with His tag at N-terminal was expressed in E. coli
Description
Staphylococcal Sortase A is a bacterial transpeptidase that covalently attaches proteins to the bacterial cell wall by cleaving between threonine and glycine at an LPXTG recognition motif and catalyzes the formation of an amide bond between the carboxyl-group of threonine and the amino-group of the cell-wall peptidoglycan. This chemistry has been exploited as a molecular "stapler" to site-specifically link proteins with the C-terminal LPETGX motif to other proteins or molecules possessing a glycine or aminomethylene motif. This reaction is applicable to proteins in solution and on the living cell surface.
Source
E. coli
Tag
His
Form
40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 8 mM imidazole, 0.04% Tween-20, and 20% glycerol
Molecular Mass
27.1 kDa
Purity
Greater than 87%
Applications
Suitable for use with in vitro protein ligation reactions.
Storage
>6 months at -80 centigrade. Avoid freeze/thaw cycles.
Official Symbol
Synonyms
class A sortase SrtA
Gene ID
Figure 1
srtA-158S, 1.jpg
