Cat.No.
Protein A-8149S
Species
Staphylococcus aureus
Product Name
Recombinant Staphylococcus aureus Protein A protein, Biotin/His-tagged
Product Overview
Recombinant Staphylococcus aureus Protein A labeled with biotin and fused with the polyhistidine tag at the N-terminus consisting 299 amino acids was expressed in E. coli
Source
E. coli
Tag
Biotin/His
Form
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally Trehalose are added as protectants before lyophilization.
Molecular Mass
DTT-reduced protein migrates as a 33.8 kDa polypeptide .
Protein length
299 amino acids
Endotoxin
Less than 1.0 EU per μg of the Biotinylated Protein A by the LAL method.
Purity
>95% as determined by reduced SDS-PAGE.
Applications
The primary amine of Biotinylated Protein A was labeled by biotin for detection in ELISA, dot blot or western blot using streptavidin or avidin-conjugated probes.
Storage
Avoid repeated freeze-thaw cycles.No activity loss was observed after storage at:In lyophilized state for 1 year (4 centigrade); After reconstitution under sterile conditions for 3 months (-70 centigrade).
