Cat.No.
HSP104-1258S
Species
Saccharomyces cerevisiae
Product Name
Recombinant Saccharomyces Cerevisiae HSP104
Product Overview
Recombinant Saccharomyces Cerevisiae HSP104 is a single, non-glycosylated polypeptide chain containing 908 amino acids and having a molecular mass of 102 kDa. Hsp104 was cloned into an E. coli expression vector and was purified to apparent homogeneity by using conventional column chromatography techniques.
Description
HSP104 is a molecular chaperone required for stress tolerance and for maintenance of [psi(+)] prions in the budding yeast Saccharomyces cerevisiae. Hsp104 can protect yeast cells against high temperature and high concentration of ethanol but mutation studies have shown this protein is not required for normal growth.
Source
Saccharomyces cerevisiae
Form
The HSP-104 protein solution contains 20mM Tris-HCl, pH 7.4,100mM NaCl. 2mM EDTA and 5% Glycerol.
Purity
Greater than 90.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
Synonyms
YLL026W; Heat shock protein that cooperates with Ydj1p (Hsp40) and Ssa1p (Hsp70) to refold and reactivate previously denatured, aggregated proteins; responsive to stresses including: heat, ethanol, and sodium arsenite; involved in [PSI+] propagation; Heat shock protein that cooperates with Ydj1p (Hsp40) and Ssa1p (Hsp70) to refold and reactivate previously denatured, aggregated proteins; responsive to stresses including: heat, ethanol, and sodium arsenite; involved in [PSI+] propagation Hsp104p
Gene ID
Protein Refseq
Chromosome Location
XII
Stability
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles.
Physical Appearance
Sterile filtered colorless solution.
Formulation
The HSP-104 protein solution contains 20mM Tris-HCl, pH 7.4,100mM NaCl. 2mM EDTA and 5% Glycerol.
