Cat.No.
GAL4-8480S
Species
Saccharomyces cerevisiae
Product Name
Recombinant Saccharomyces Cerevisiae GAL4, His-tagged
Product Overview
Recombinant Saccharomyces Cerevisiae GAL4, fused with His tag, was expressed in E. coli.
Description
Transcriptional activity is greatly stimulated by promoter-specific activator proteins . These are modular proteins, consisting of a DNA-binding domain and a regulatory (activator) domain . The GAL4 protein of yeast activates the transcription of several genes involved in galactose metabolism. This event requires that GAL4 bind to upstream activation sites with the consensus sequence 5-CGGN5(T/A)N5CCG-3 . A fragment of the GAL4 protein, comprising amino acids 1-147, binds DNA but fails to activate transcription . Linking of an acidic synthetic peptide, forming an ?-helix (AH), to this GAL4 DNA-binding domain, results in a protein with an amphipathic structure . This fusion protein is able to activate transcription of a gene, bearing the GAL4 binding sites in an in vitro transcription system by targeting TFIIB in the pre-initiation complex.
Source
E. coli
Tag
His
Form
20 mM Tris-Cl, pH 7.9, 20% Glycerol, 100 mM KCl, 1 mM DTT and 0.2 mM EDTA
AA Sequence
MKLLSSIEQA CDICRLKKLK CSKEKPKCAK CLKNNWECRY SPKTKRSPLT RAHLTEVESR LERLEQLFLL IFPREDLDMI LKMDSLQDIK ALLTGLFVQD NVNKDAVTDR LASVETDMPL TLRQHRISAT SSSEESSNKG QRQLTVSELN ELNELNALLN NN
Purity
>95% as determined by SDS-PAGE
Storage
-80 °C
Official Symbol
GAL4
Synonyms
GAL4; GAL81; Gal4p;
UniProt ID
Concentration
300 μg/ml
Bio-activity
1 unit equals 1 ng of purified protein. 20 units are sufficient for reconstituted transcription assay and 100 units are sufficient for a protein-protein interaction assay.
Gene ID
mRNA Refseq
Protein Refseq
Chromosome Location
chromosome: XVI
Sequence
MKLLSSIEQA CDICRLKKLK CSKEKPKCAK CLKNNWECRY SPKTKRSPLT RAHLTEVESR LERLEQLFLL IFPREDLDMI LKMDSLQDIK ALLTGLFVQD NVNKDAVTDR LASVETDMPL TLRQHRISAT SSSEESSNKG QRQLTVSELN ELNELNALLN NN
Shipping
Dry Ice
