Cat.No.
KEX2-01S
Species
Saccharomyces cerevisiae
Product Name
Recombinant S. cerevisiae KEX2 Protein
Product Overview
Recombinant Kex2 protease is a genetically engineered protein expressed in Pichia pastoris and with equivalent properties compared to native Saccharomyces cerevisiae Kex2.
Description
Processing of precursors of alpha-factors and killer toxin.
Source
Yeast
Form
White or off white powder
Molecular Mass
67.0±6.7kDa
Purity
Single major band
Storage
Store at 2-8 centigrade
Official Symbol
Synonyms
KEX2; kexin KEX2; Endoproteinase Lys/Arg-Arg; Kex2 Protease; YSCF Protenase; 3.4.21.61; QDS1; SRB1; VMA45; kexin KEX2; EC 3.4.21.61; Kexin, a subtilisin-like protease (proprotein convertase); a calcium-dependent serine protease involved in the activation of proproteins of the secretory pathway
UniProt ID
Bio-activity
≥10.0 units/mg pro.
The activity of Kex2 is not affected by the conventional serine protease inhibitors such as PMSF, TPCK, TLCK inhibition.
The activity of Kex2 is not affected by the conventional serine protease inhibitors such as PMSF, TPCK, TLCK inhibition.
Usage
Recommended reaction buffer: pH 7.0-9.0, 50 mM Tris-HCl, 2 mM Ca2+ or HEPES, 5 mM Ca2+. If it is not used immediately after dissolving, it is recommended to dissolve the powder with 20 mM (pH 5.2) NaAc-HAc and 2 mM Ca2+ buffer to make the final concentration is about 1-10 mg/mL, then make suitable packing as your requirement and store at -20 centigrade. Reaction was carried out, using pH 7.0-9.0, 50 mM Tris-HCl, 2 mM Ca2+ or HEPES, 5 mM Ca2+ buffer.
Note: The optimum reaction pH of the enzyme is pH 9.0 and the optimum stable pH is 5.0-6.0.
Note: The optimum reaction pH of the enzyme is pH 9.0 and the optimum stable pH is 5.0-6.0.
Unit Definition
One unit of Kex2 activity will release 1μmol 4-nitroaniline per minute in a reaction volume of 3.0 mL at pH 8.0 and 25 centigrade with Boc-QRR-pNA (Boc-Gln-Arg-Arg-pNA) as the substrate.
Gene ID
mRNA Refseq
Protein Refseq
Stability
Stability of storage: Stored under 2-8 centigrade in sealed container. It is stable within 12 months. After dissolved with 20mM NaAc-HAc, pH 5.2, 2mM Ca2+, it should be stored under -20 centigrade.
Stability of transport: The product is stable by blue ice insulation transport.
Stable in buffer (pH 5.0-6.0).
Stability of transport: The product is stable by blue ice insulation transport.
Stable in buffer (pH 5.0-6.0).
Optimum pH
9.0
Characteristic
Animal origin free: Recombinant Kex2 protease is no exogenous virus contamination, and any animal origin material is not used in the production process.
Stable quality: Mass production can ensure stable and continuous batch production. It is no difference between the batch and the product quality is stable.
High purity: Higher specific activity. Host protein residues is less than the limits of biological products.
Lyophilized powder: The product is lyophilized powder and is easy to store and transport.
Compliance with regulatory requirements: Production equipment and production environment comply with relevant regulatory requirements, and the production process is in full compliance with NSF ISO 9001: 2015 quality system and GMP guidelines.
Complete quality documents: we can provide relevant regular support files in according to customers’ requirement.
Stable quality: Mass production can ensure stable and continuous batch production. It is no difference between the batch and the product quality is stable.
High purity: Higher specific activity. Host protein residues is less than the limits of biological products.
Lyophilized powder: The product is lyophilized powder and is easy to store and transport.
Compliance with regulatory requirements: Production equipment and production environment comply with relevant regulatory requirements, and the production process is in full compliance with NSF ISO 9001: 2015 quality system and GMP guidelines.
Complete quality documents: we can provide relevant regular support files in according to customers’ requirement.
