Cat.No.
SAG1197-835S
Species
S. agalactiae
Product Name
Recombinant S. agalactiae SAG1197 Protein, Met & His-tagged
Product Overview
Recombinant S. agalactiae SAG1197 (Accession # NP_688206) Ser259-Ile1072, fused with an N-terminal Met and 6-His tag, was produced in E. coli
Source
E. coli
Tag
His
Predicted N Terminal
Met
Form
Supplied as a 0.2 µm filtered solution in Tris and NaCl.
Bio-activity
Measured by its ability to cleave the native substrate Hyaluronan.The specific activity is >75,000 pmol/min/µg, as measured under the described conditions.
Molecular Mass
Recombinant S. agalactiae SAG1197, Met & His-tagged has a calculated MW of 93 kDa. In SDS-PAGE migrates as 86 kDa, reducing conditions.
Protein length
Ser259-Ile1072
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Storage
Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).
Reconstitution
Reconstitute at 10 µg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Official Symbol
SAG1197
Synonyms
SAG1197
Gene ID
Protein Refseq
