Cat.No.
PPA-60S
Species
Sulfolobus acidocaldarius
Product Name
Recombinant S. acidocaldarius Thermostable Pyrophosphatase
Product Overview
A recombinant E. coli strain carrying the Thermostable Pyrophosphatase gene from S. acidocaldarius.
Description
Thermostable pyrophosphatase is a recombinant enzyme from Sulfolobus acidocaldarius which catalyzes the Mg-dependent reaction of P2O7-4 + H2O → 2HPO4-2. It is has a low Km (5.4 uM) for pyrophosphate, is active between pH 7 and 9, has an optimal temperature for activity at 75°C and is functional under PCR conditions.
Source
E. coli
Molecular Mass
19,381 Daltons
Purity
>95% by SDS-PAGE
Storage
-25 to -15°C
Concentration
2,000 U/ml
Unit Definition
One unit is the amount of enzyme that will liberate 1 μmol of phosphate per minute from inorganic pyrophosphate at 75°C and pH 8.5
Storage Buffer
Supplied in: 10 mM Tris-HCl pH 7.5, 50 mM NaCl, 50% Glycerol, 1 mM DTT, 0.1 mM EDTA
Bio-activity
3,500 U/mg
