Cat.No.
RFP-005E
Species
E. coli
Product Name
Recombinant Red Fluorescent Protein, His-tagged
Product Overview
The recombinant RFP (dsRed) was expressed and purified from transformed E. coli using a method that ensures high purity and maximal RFP fluorescence. Endotoxin was removed, so the protein is suitable for in vivo injection or cell culture applications. The protein is a 27.6 kDa monomers containing a N-terminus 6x His-Tag with Ex./Em. = 557/585 nm, extinction coefficient (e 280 nm = 34380 M^-1 Cm^-1).
Fluorescent protein ideal for subcellular labeling/visualization
Fluorescent protein ideal for subcellular labeling/visualization
Description
Red fluorescent protein (RFP) is a fluorophore that fluoresces red-orange when excited. Several variants have been developed using directed mutagenesis. The original was isolated from Discosoma, and named DsRed. Others are now available that fluoresce orange, red, and far-red.
Source
E. coli
Tag
His
Form
Lyophilized (Freeze Dried)
Molecular Mass
27.6 kDa
Endotoxin
<0.1 ng/μg
Purity
≥97%
Applications
The protein is suitable as control reagent for dsRed expression studies or as labeling reagent. Applications include: Use as standards for SDS-PAGE, Western blot analysis, label other proteins, calibration of fluorometers, flow cytometers, and fluorescence microscope, as well as microinjection of dsRed into cells and tissues, etc. The recombinant dsRed is ideal for fusion tag applications and is perfect for dual labeling with EGFP.
Usage
For Research Use Only! Not to be used in humans
Storage
At -20 centigrade.
At -80 centigrade for long-term storage.
At -80 centigrade for long-term storage.
Reconstitution
Reconstitute with dH₂O to 1 mg/mL
Handling
Centrifuge the vial prior to opening.
Synonyms
RFP, Red Fluorescent Protein
