Cat.No.
RNASEH2-01
Species
Pyrococcus abysii
Product Name
Recombinant Pyrococcus abysii RNASEH2 protein
Product Overview
Recombinant Pyrococcus abysii RNASEH2 was expressed in E. coli.
Description
RNase H2 Enzyme is a recombinant endoribonuclease that binds to RNA-DNA duplexes, and cleaves the RNA strand leaving a 5’phosphate and a 3’hydroxyl group. The RNase H2 enzyme differs from RNase H1 in that RNase H2 will cleave at a single ribonucleotide residue embedded within a heteroduplex. RNase H2 will not cleave single-stranded RNA.
Source
E. coli
Molecular Mass
27,573.6 daltons
Storage
Storage at -20 centigrade in low protein binding tubes.
Unit Definition
One enzymatic unit is the amount of enzyme needed to cleave 1 nmole of the DNA-RNA-DNA heteroduplex substrate S-rC14-1-15 per minute at 70 centigrade in Mg++ Cleavage Buffer (10 mM Tris-HCl pH 8.0, 50 mM NaCl, 4 mM MgCl2, 10μg/mL BSA)
5’ CTCGTGAGGTGATGcAGGAGATGGGAGGCG 3’
3’ GAGCACTCCACTACGTCCTCTACCCTCCGC 5’
5’ CTCGTGAGGTGATGcAGGAGATGGGAGGCG 3’
3’ GAGCACTCCACTACGTCCTCTACCCTCCGC 5’
