Cat.No.
Protein L-3141P
Species
Peptostreptococcus magnus
Product Name
Recombinant Peptostreptococcus magnus Protein L protein, His-tagged
Product Overview
Recombinant Peptostreptococcus magnus Protein L fused with the polyhistidine tag at N-terminus and a single cysteine at C-terminus was expressed in E. coli
Source
E. coli
Tag
His
Form
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4.
Bio-activity
Measured by its binding ability in a functional ELISA.Immobilized Immobilized Human IgG1 containing Kappa L chain at 5 μg/mL (100 μL/well) can bind Recombinant Protein L Protein.The EC50 is typically 0.1-1 ng/mL.
Molecular Mass
DTT-reduced protein migrates as a 36-38 kDa polypeptide .Protein L Protein can bind to VL-Kappa.
Endotoxin
Less than 1.0 EU per μg of the Protein L by the LAL method.
Purity
>98% as determined by SDS-PAGE.
Storage
Avoid repeated freeze-thaw cycles.No activity loss was observed after storage at:In lyophilized state for 1 year (4 centigrade); After reconstitution under sterile conditions for 3 months (-70 centigrade).
