Cat.No.
Protein L-8141P
Species
Peptostreptococcus magnus
Product Name
Recombinant Peptostreptococcus magnus Protein L protein, Biotin/His-tagged
Product Overview
Recombinant Peptostreptococcus magnus Protein L protein labeled with specially optimized long spacer biotin reagent and fused with the polyhistidine tag at N-terminus and a single cysteine at C-terminus was expressed in E. coli
Source
E. coli
Tag
Biotin/His
Form
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally Trehalose are added as protectants before lyophilization.
Bio-activity
Measured by its binding ability in a functional ELISA. Immobilized Human IgG1 containing Kappa L chain at 5 μg/mL (100 μL/well) can bind Biotinylated Protein L Superior Sensitivity. The EC50 is typically 0.5-5 ng/mL.
Molecular Mass
DTT-reduced protein migrates as a 42-47 kDa polypeptide
Endotoxin
Less than 1.0 EU per μg of the Biotinylated Protein L by the LAL method.
Purity
>95% as determined by reduced SDS-PAGE.
Applications
Biotinylated Protein L can bind to VL-Kappa. The primary amine of Biotinylated Protein L was chemically labeled by specially optimized long spacer biotin reagent for detection in ELISA, dot blot or western blot using streptavidin or avidin-conjugated prob
Storage
Avoid repeated freeze-thaw cycles.No activity loss was observed after storage at:In lyophilized state for 1 year (4 centigrade); After reconstitution under sterile conditions for 3 months (-70 centigrade).
