Cat.No.
toxA-001P
Species
P. multocid
Product Name
Recombinant P. multocida toxA Protein, his-tagged
Product Overview
This product is derived from a recombinant P. multocida ToxA with His6-tag at N-terminal expressed in E. coli
Description
Pasteurella multocida toxin (PMT) is produced by a gram-negative bacillus, Pasteurella multocida. PMT activates the Gq and G12/13 dependent signaling pathways. Gq and G12/13 are alpha subunits of the GTP trimer bound protein of animal cells. It does not activate the highly related G11-dependent pathways. This toxin binds to a ganglioside-type cell surface receptor, acts intracellularly after having been internalized through an endocytic pathway, and has pleiotropic effects on cell physiology. Therefore, it does not function on receptor-deficient cells or cells defective in the endocytosis pathway.
Source
E. coli
Tag
His
Form
50% glycerol, 5mM Tris-HCl (pH7.5), 0.1M NaCl
Molecular Mass
145 kDa
Purity
More than 90% purity by SDS-PAGE (CBB staining)
Applications
1) For the studies on the GTP trimer bound protein- dependent signaling pathway.
2) As a PMT antigen for immunological assays such as ELISA, western blotting and dot blotting.
2) As a PMT antigen for immunological assays such as ELISA, western blotting and dot blotting.
Storage
Ship at 4centigrade or -20centigrade and store at -20centigrade (or -80centigrade for long period).
Warning
Wear safety glasses, protective clothing, and rubber or latex gloves. and when handling hypodermic needles avoid inadvertent selfinoculation. Do not pipette by mouth. Avoid inhalation of this product. This product is to be used by skilled personnel in a laboratory setting only. Good laboratory technique should be employed. This product is for research purposes only. It is not for use in humans and is not to be used as a diagnostic agent.
Gene Name
Official Symbol
Synonyms
toxA; dermonecrotic toxin
Gene ID
Protein Refseq
UniProt ID
Figure 1
toxA-001P, 1.jpg
Figure Title 1
SDS-polyacrylamide gel electrophoresis of PMT
Figure 2
toxA-001P, 2
Figure Title 2
Assay of Biological activity of PMT: Mouse embryonic cells (Swiss 3T3) were treated with PMT at 10 ng/ml at 37centigrade for 18 h.
Left: untreated cells. Right: Treated cells. Foci formation by aggregation of cells was observed.
Left: untreated cells. Right: Treated cells. Foci formation by aggregation of cells was observed.
