Cat.No.
RELG-2216M
Species
Mycobacterium Tuberculosis
Product Name
Recombinant Mycobacterium Tuberculosis RELG Protein (1-87 aa), His-SUMO-tagged
Product Overview
Recombinant Mycobacterium Tuberculosis (strain ATCC 25618/H37Rv) RELG Protein (1-87 aa) is produced by E. coli expression system. This protein is fused with a 6xHis-SUMO tag at the N-terminal. Protein Description: Full Length.
Description
Toxic component of a toxin-antitoxin (TA) module. Has RNase activity and preferentially cleaves at the 3'-end of purine ribonucleotides. Overexpression in M.tuberculosis or M.smegmatis inhibits colony formation in a bacteriostatic rather than bacteriocidal fashion. Its toxic effect is neutralized by coexpression with cognate antitoxin RelB2 (shown only for M.smegmatis). Overexpression also increases the number of gentamicin-tolerant and levofloxacin-tolerant persister cells.
Source
E. coli
Tag
His/SUMO
Form
Tris-based buffer,50% glycerol
Molecular Mass
26.2 kDa
Protein length
1-87 aa
AA Sequence
MPYTVRFTTTARRDLHKLPPRILAAVVEFAFGDLSREPLRVGKPLRRELAGTFSARRGTYRLLYRIDDEHTTVVILRVDHRADIYRR
Purity
> 90% as determined by SDS-PAGE.
Storage
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 centigrade/-80 centigrade. The shelf life of lyophilized form is 12 months at -20 centigrade/-80 centigrade.
Concentration
A hardcopy of COA will be sent along with the products. Please refer to it for detailed information.
Official Symbol
Synonyms
relG; Putative endoribonuclease RelG; relE2;
Gene ID
Protein Refseq
UniProt ID
Figure 1
RELG-2216M, 1.jpg
Figure Note 1
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
