Cat.No.
groEL2-33M
Species
Mycobacterium Tuberculosis
Product Name
Recombinant Mycobacterium Tuberculosis groEL2 protein, His-tagged
Product Overview
Recombinant Mycobacterium Tuberculosis groEL2 fused with His tag at N-terminal was expressed in E. coli
Description
Heat shock proteins induce pro-inflammatory cytokines. Mycobacterial HSPs participate in cytokine expression resulting from infection by M. tuberculosis. Furthermore, HSPs stabilize cellular proteins in response to various sources of stress or injury. HSP65 is one of the most essential defending immunogens against the tuberculosis infection. HSP65 is presented to human CD41 T cells in association with multiple HLA-DR molecules. The M. tuberculosis HSP65 signals through TLR4.
Source
E. coli
Tag
His
Form
The HSP65 protein was lyophilized from a concentrated (1mg/ml) solution containing 10mM Na-phosphate pH-7.4, 130mM NaCl and 2.5mM KCl.
Molecular Mass
57.4 kDa
AA Sequence
HHHHHHGSAKTIAYDEEARRGLERGLNALADAVKVTLGPKGRNVVLEKKWGAPTITNDGVSIAKEIELEDPYEKI GAELVKEVAKKTDDVAGDGTTTATVLAQALVREGLRNVAAGANPLGLKRGIEAVEKVTETLLKGAKEVETKEQI AATAAISAGDQSIGDLIAEAMDKVGNEGVITVEESNTFGLQLELTEGMRFDKGYISGYFVTDPERQEAVLEDPY ILLVSSKVSTVKDLLPLLEKVIGAGKPLLIIEDVEGEALSTLVVNKIRGTFKSVAVKAPGFGDRRKAMLQDMAIL TGGQVISEEVGLTLENADLSLLGKARKVVVTKDETTIVEGAGDTDAIAGRVAQIRQEIENSDSDYDREKLQERL AKLAGGVAVKAGAATEVELKERKHRIEDAVRNAKAAVEEGIVAGGGVTLLQAAPTLDELKLEGDEATGANIVKVA LEAPLKQIAFNSGLEPGVVAEKVRNLPAGHGLNAQTGVYEDLLAAGVADPVKVTRSALQNASIAGLFLTTEAVV ADKPEKEKASVPGGGDMGGMDF
Purity
Greater than 95.0% as determined by SDS-PAGE.
Stability
Lyophilized HSP65 although stable at room temperature for 3 weeks, should be stored desiccated below -18 centigrade. Upon reconstitution HSP65 should be stored at 4 centigrade between 2-7 days and for future use below -18 centigrade. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.
Storage
Storage at -70 centigrade
Reconstitution
It is recommended to reconstitute the lyophilized HSP-65 in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Official Symbol
Synonyms
groEL-2; groL2; hsp60; hsp65; molecular chaperone GroEL
Gene ID
Protein Refseq
UniProt ID
