Cat.No.
85C-895M
Species
Mycobacterium tuberculosis
Product Name
Recombinant Mycobacterium tuberculosis 85C protein, His-tagged
Product Overview
C-terminal 6xHis fusion protein of antigen 85-C (Rv0129c) (M. Tuberculosis/H37Rv)(a.a.1-344) (Genbank Accession No. NP_334547). Predicted to be an esterase.
Description
Mycobacterium tuberculosis is an obligate pathogenic bacterial species in the family Mycobacteriaceae and the causative agent of tuberculosis. First discovered in 1882 by Robert Koch, M. tuberculosis has an unusual, waxy coating on its cell surface primarily due to the presence of mycolic acid. This coating makes the cells impervious to Gram staining, and as a result, M. tuberculosis can appear either Gram-negative or Gram-positive. Acid-fast stains such as Ziehl-Neelsen, or fluorescent stains such as auramine are used instead to identify M. tuberculosis with a microscope. The physiology of M. tuberculosis is highly aerobic and requires high levels of oxygen. Primarily a pathogen of the mammalian respiratory system, it infects the lungs. The most frequently used diagnostic methods for tuberculosis are the tuberculin skin test, acid-fast stain, culture, and polymerase chain reaction.
Source
E. coli
Tag
His
Form
Each vial contains 100 µg of lyophilized protein in PBS with 8M Urea. Reconstitute the protein with 100 µl of Millipore water.
Protein length
aa1-344
Purity
>95%, based on SDS PAGE
Applications
WB standard, antibody ELISA, immunogen, etc.
Storage
Before reconstitution, stable for 1 year at -20°C from the date of shipment. After reconstitution, stable for a month at 4°C. Nonhazardous. No MSDS required.
