Cat.No.
NA-474M
Species
M. viridifaciens
Product Name
Recombinant M.viridifaciens neuraminidase NA Protein, His-tagged
Product Overview
Recombinant M.viridifaciens neuraminidase (BAA00852.1) (Pro42-Gly403) was expressed, with a N-terminal polyhistidine tag
Source
E. coli
Tag
His
Predicted N Terminal
Met 1
Form
Lyophilized from sterile PBS, pH 7.4. Normally 5 %- 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Bio-activity
Measured by its ability to cleave a fluorogenic substrate, 2'-(4-Methylumbelliferyl)-α-D-N-acetylneuraminic acid. The specific activity is 10,000 pmoles/min/μg. as measured under the described conditions.
Molecular Mass
The recombinant M.viridifaciens neuraminidase consisting of 369 amino acids and has a calculated molecular mass of 39.7 kDa as estimated in SDS-PAGE under reducing conditions as predicted.
Protein length
Pro42-Gly403
Endotoxin
< 1.0 EU per μg of the protein as determined by the LAL method
Purity
> 97 % as determined by SDS-PAGE
Storage
Samples are stable for up to twelve months from date of receipt at-70 centigrade. Store it under sterile conditions at-20 centigrade to-80 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents.
Official Symbol
NA
Synonyms
neuraminidase;NA Protein
