Cat.No.
PNGF-200F
Species
F. meningosepticum
Product Name
Recombinant Mouse Hhip Protein
Product Overview
Recombinant F. meningosepticum PNGF (Accession # AAA85323) Ala41-Asn354, fused with N-terminal Met and 6-His tag, was produced in E. coli-derived.
Source
E. coli
Tag
His
Predicted N Terminal
Met
Form
Supplied as a 0.2 µm filtered solution in Tris and NaCl.
Bio-activity
Measured by its ability to deglycosylate ribonuclease B under denatured conditions.>50% ribonuclease B (10 µg) is deglycosylated by 10 ng of rFmPNGase F within 30 minutes, as measured under the described conditions.
Molecular Mass
Recombinant F. meningosepticum PNGF, Met&His-tagged has a calculated MW of 36 kDa. In SDS-PAGE migrates as 33-35 kDa, reducing conditions.
Purity
>90%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain at 5 µg per lane.
Storage
Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).
