Cat.No.
UDG-59B
Species
Marine bacteria
Product Name
Recombinant Marine bacteria thermolabile Uracil DNA Glycosylase
Product Overview
A recombinant E. coli strain carrying the recombinant thermolabile Uracil DNA Glycosylase gene isolated from a marine bacteria.
Description
Thermolabile Uracil-DNA Glycosylase removes uracil from DNA by hydrolyzing the N-glycosylic bond between the deoxyribose and the base leaving an AP (apurinic or apyrimidinic) site. This enzyme ( 1-10 units) is completely inactivated by a 10 minute incubation at temperatures greater than 50°C in the 1x reaction buffer as measured in the unit characterization assay.
Source
E. coli
Bio-activity
30,000 U/mg
Molecular Mass
26,218 Daltons
Purity
>98% by SDS-PAGE
Unit Definition
1 unit is defined as the amount of Thermolabile UDG required to release 1nmol of Uracil from dU-containing DNA in one hour at 37°C.
Storage
-25 to -15°C
Concentration
1,000 U/ml
Storage Buffer
Supplied in: 50mM Tris-HCl, 50mM NaCl, 1mM DTT, 0.1mM EDTA, 50% glycerol pH 7.5 @ 25°C. Supplied with: 10X Reaction Buffer: 700mM Tris-HCl, 100mM NaCl, 10mM EDTA, 1 mg/mL BSA pH 8.0 @ 25°C
