Cat.No.
CED41
Species
Lysobacter sp.
Product Name
Recombinant Lysobacter Enzymogenes Arg-C, His-tagged
Product Overview
Recombinant Lysobacter Enzymogenes Arg-C is a 26.8 kDa protease consisting of 252 amino acid residues including a C-terminal His-Tag.
Description
Proteases (also called Proteolytic Enzymes, Peptidases, or Proteinases) are enzymes that hydrolyze the amide bonds within proteins or peptides. Most proteases act in a specific manner, hydrolyzing bonds at or adjacent to specific residues or a specific sequence of residues contained within the substrate protein or peptide. Proteases play an important role in most diseases and biological processes including prenatal and postnatal development, reproduction, signal transduction, the immune response, various autoimmune and degenerative diseases, and cancer. They are also an important research tool, frequently used in the analysis and production of proteins.
Source
Hi-5 Insect cells
Tag
His
Bio-activity
The reaction is measured as an increase in absorbance at 253 nm resulting from the hydrolysis of N-benzoyl-L-arginine ethyl ester (BAEE).
AA Sequence
GVGDIGSSDY CEKDIVCRVK PSAEFLSASK SVARMVFTPK TGYTGYCSGT LLNNSNSPKR QLFWSAAHCI STQKVANTLQ TYWLYDATGC DNDTLSDKAV TLTGGATLLH SHATRDTLLL ELKSAPPSGA YYAGWNSSAI ATKGTAIEGI HHPSGDLKKY SLGSVTALSS TIDGKKPLTK VAWTTGVTEG GSSGSGLFTI SSTSGYQLRG GLYGGTSYCS APSDPDYYSQ LDGVWSSIKT YFSPHHHHHH HH
Purity
Greater than 98% by SDS-PAGE gel and HPLC analyses.
Storage
Store at -20°C. Avoid repeated freeze-thaw cycles.
