Cat.No.
Heparinase III-205P
Species
P. heparinus
Product Name
Recombinant Human DLL1 Protein
Product Overview
Recombinant P. heparinus Heparinase III (Accession # AAB18278) Gln25-Pro659, fused with an N-terminal Met and 6-His tag, was produced in E. coli-derived.
Source
E. coli
Tag
His
Predicted N Terminal
Met
Form
Supplied as a 0.2 µm filtered solution in Tris, NaCl and Glycerol.
Bio-activity
Measured by its ability to liberate oligosaccharides from heparin.The specific activity is >3,000 pmol/min/µg, as measured under the described conditions. See Activity Assay Protocol on www.RnDSystems.com.
Molecular Mass
Recombinant P. heparinus Heparinase III, Met&His-tagged has a calculated MW of 74 kDa. In SDS-PAGE migrates as 66 kDa, reducing conditions.
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain at 5 µg per lane.
Storage
Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).
