Cat.No.
COP23
Species
Others
Product Name
Recombinant Golden Nuclease
Product Overview
GoldenNuclease is a recombinant form of Serratia macescens extracellularendonuclease (encoded by the same gene of Benzonase) produced in Escherichiacoli cells.
Description
GoldenNuclease is a recombinant form of Serratia macescens extracellularendonuclease (encoded by the same gene of Benzonase) produced in E. coliusing a proprietary process. This nonspecific endonuclease hydrolyzes bothsingle- and double-stranded nucleic acids (DNA and RNA) to 5"-phosphorylatedoligonucleotides of 1-4 bases in length. TurboNuclease is a highly purifiedhomodimer of 27 kDa subunits that has exceptional high specific activity andis free of protease activity. It is ideal to digest nucleic acids and toreduce viscosity during protein purification and sample preparation.
Source
E. coli
Form
Solutionin 50% glycerol containing 20 mM Tris HCl, pH 8.0, 2 mM MgCl2, and 20 mMNaCl.
Unit Definition
One unit will digest sonicated salmon sperm DNA toacid-soluble oligonucleotides equivalent to a ΔA260 of 1.0 in 30 min at pH8.0 at 37 ℃(reaction volume 2.625 ml).
Storage
Storeat -20℃ upon received. Avoid repeated freeze-thaw cycles.
Concentration
>250units/μL
