Cat.No.
RPSG-1224E
Species
E. coli
Product Name
Recombinant Escherichia coli RPSG Protein (30S) (2-179 aa), His-tagged
Product Overview
Recombinant Escherichia coli (strain K12) RPSG Protein (30S) (2-179 aa) is produced by E. coli expression system. This protein is fused with a 6xHis tag at the N-terminal. Protein Description: Full Length of Mature Protein.
Description
One of the primary rRNA binding proteins, it binds directly to 16S rRNA where it nucleates assbly of the head domain of the 30S subunit. Is located at the subunit interface close to the decoding center, where it has been shown to contact mRNA. Has been shown to contact tRNA in both the P and E sites; it probably blocks exit of the E site tRNA.
Source
E. coli
Tag
His
Form
Tris-based buffer,50% glycerol
Molecular Mass
23.9 kDa
Protein length
2-179 aa
AA Sequence
PRRRVIGQRKILPDPKFGSELLAKFVNILMVDGKKSTAESIVYSALETLAQRSGKSELEAFEVALENVRPTVEVKSRRVGGSTYQVPVEVRPVRRNALAMRWIVEAARKRGDKSMALRLANELSDAAENKGTAVKKREDVHRMAEANKAFAHYRWLSLRSFSHQAGASSKQPALGYLN
Purity
> 90% as determined by SDS-PAGE.
Storage
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 centigrade/-80 centigrade. The shelf life of lyophilized form is 12 months at -20 centigrade/-80 centigrade.
Concentration
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
Official Symbol
Synonyms
ECK3328;
Gene ID
Protein Refseq
UniProt ID
Figure 1
RPSG-1224E, 1.jpg
Figure Note 1
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
