Cat.No.
RPLF-1237E
Species
E. coli
Product Name
Recombinant Escherichia coli RPLF Protein (50S) (2-175 aa), GST-tagged
Product Overview
Recombinant Escherichia coli (strain K12) RPLF Protein (50S) (2-175 aa) is produced by E. coli expression system. This protein is fused with a GST tag at the N-terminal. Protein Description: Partial.
Description
This protein binds directly to at least 2 domains of the 23S ribosomal RNA, thus is important in its secondary structure. It is located near the subunit interface in the base of the L7/L12 stalk, and near the tRNA binding site of the peptidyltransferase center.Gentamicin-resistant mutations in this protein affect translation fidelity.
Source
E. coli
Tag
GST
Form
Tris-based buffer,50% glycerol
Molecular Mass
45.5 kDa
Protein length
2-175 aa
AA Sequence
SRVAKAPVVVPAGVDVKINGQVITIKGKNGELTRTLNDAVEVKHADNTLTFGPRDGYADGWAQAGTARALLNSMVIGVTEGFTKKLQLVGVGYRAAVKGNVINLSLGFSHPVDHQLPAGITAECPTQTEIVLKGADKQVIGQVAADLRAYRRPEPYKGKGVRYADEVVRTKEAK
Purity
> 90% as determined by SDS-PAGE.
Storage
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 centigrade/-80 centigrade. The shelf life of lyophilized form is 12 months at -20 centigrade/-80 centigrade.
Concentration
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
Official Symbol
Synonyms
ECK3292;
Gene ID
Protein Refseq
UniProt ID
Figure 1
RPLF-1237E, 1.jpg
Figure Note 1
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
