Cat.No.
EGFP-003E
Species
E. coli
Product Name
Recombinant Enhanced Green Fluorescent Protein, His-tagged
Product Overview
The recombinant EGFP is expressed and purified from transformed E. coli using a method that ensures high purity and maximal GFP fluorescence. Endotoxin has been removed, so the protein is suitable for in vivo injection or cell culture applications. The recombinant EGFP is a 32.7 kDa monomer with 293 amino acids tagged with 6XHis on both N- and C-terminus. Therefore, the EGFP can be detected by both His-tag antibody and GFP antibody on Western Blot analysis. The excitation and emmission spectra for the recombinant EGFP is identical to GFP purified from Aequorea Victoria, with Ex/Em = 488/507 nm (ε489 nm = 55,000 M^-1 Cm^-1; ε280 nm = 21980 M^-1Cm^-1).
Fluorescent protein ideal for subcellular labeling/visualization.
Fluorescent protein ideal for subcellular labeling/visualization.
Description
Green Fluorescent Protein (GFP) is a versatile biological marker for monitoring physiological processes, visualizing protein localization, and detecting transgenic expression in vivo.
Source
E. coli
Tag
His
Form
Lyophilized (Freeze Dried)
Molecular Mass
32.7 kDa
Endotoxin
<0.1 ng/μg
Purity
≥97%
Applications
The protein is suitable as control reagent for GFP expression studies. Applications include: Use as standard for SDS-PAGE, Western blot analysis, calibration of fluorometers, flow cytometers, and fluorescence microscope, as well as microinjection of EGFP into cells and tissues, etc. The recombinant EGFP can also be conjugated to other proteins. GFP antibody is also available which recognizes the recombinant EGFP protein.
Usage
For Research Use Only! Not to be used in humans
Storage
At -20 centigrade.
At -80 centigrade for long-term storage.
At -80 centigrade for long-term storage.
Reconstitution
Reconstitute with PBS to 1 mg/mL
Handling
Centrifuge the vial prior to opening.
Synonyms
E-GFP, Enhanced Green Fluorescent Protein
