Cat.No.
zwf-001E
Species
E. coli
Product Name
Recombinant E.coli zwf protein
Product Overview
Recombinant E.coli G7PD (1-491aa) protein without tag was expressed in E. coli.
Description
Residues 199-203 of zwf encode a NNWDN peptide involved in ChpA(MazF) toxicity that is likely converted to NNWNN by AsnA; a zwf deletion prevents NNWNN activity (Kolodkin-Gal, 2007). [More information is available at EcoGene: EG11221]. A null mutation in the zwf gene encoding glucose 6-phosphate-1-dehydrogenase does not affect the growth rate significantly.
Source
E. coli
Form
Liquid
Bio-activity
Specific activity is >50 unit/mg obtained by measuring the increase of beta-NADPH in absorbance at 340 nm resulting from the reduction of beta-NADP. One unit oxidizes 1.0 umole D-glucose-6-phosphate to 6-phospho-D-gluconate per min in the presence of beta-NADP at pH 7.4 at 25 centigrade.
Molecular Mass
55.7 kDa
Protein length
1-491
AA Sequence
MAVTQTAQACDLVIFGAKGDLARRKLLPSLYQLEKAGQLNPDTRIIGVGRADWDKAAYTKVVREALETFMKETIDEGLWDTLSARLDFCNLDVNDTAAFSRLGAMLDQKNRITINYFAMPPSTFGAICKGLGEAKLNAKPARVVMEKPLGTSLATSQEINDQVGEYFEECQVYRIDHYLGKETVLNLLALRFANSLFVNNWDNRTIDHVEITVAEEVGIEGRWGYFDKAGQMRDMIQNHLLQILCMIAMSPPSDLSADSIRDEKVKVLKSLRRIDRSNVREKTVRGQYTAGFAQGKKVPGYLEEEGANKSSNTETFVAIRVDIDNWRWAGVPFYLRTGKRLPTKCSEVVVYFKTPELNLFKESWQDLPQNKLTIRLQPDEGVDIQVLNKVPGLDHKHNLQITKLDLSYSETFNQTHLADAYERLLLETMRGIQALFVRRDEVEEAWKWVDSITEAWAMDNDAPKPYQAGTWGPVASVAMITRDGRSWNEFE
Purity
>95% by SDS-PAGE
Applications
Enzyme Activity, SDS-PAGE
Storage
Can be stored at +2 to +8 centigrade for 1 week. For long term storage, aliquot and store at -20 to -80 centigrade. Avoid repeated freezing and thawing cycles.
Concentration
1 mg/mL (determined by Bradford assay)
Storage Buffer
Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol, 0.1mM PMSF, 2mM EDTA, 0.5mM DTT
Official Symbol
Synonyms
zwf; NADP(+)-dependent glucose-6-phosphate dehydrogenase; ECK1853; NADP(+)-dependent glucose-6-phosphate dehydrogenase; EC 1.1.1.49
Gene ID
Protein Refseq
UniProt ID
Figure 1
zwf-001E, 1.jpg
Figure Note 1
3 μg by SDS-PAGE under reducing condition and visualized by coomassie blue stain.
Figure Title 1
SDS-PAGE
