Cat.No.
ung-282E
Species
E. coli
Product Name
Recombinant E.coli uracil-DNA-glycosylase, His-tagged
Product Overview
ung, 1-229aa, E.coli, His tag, E.coli
Description
Ung, also known as Uracil-DNA glycosylase, is to prevent mutagenesis by eliminating uracil from DNA molecules by cleaving the N-glycosylic bond and initiating the base-excision repair (BER) pathway. Uracil bases occur from cytosine deamination or misincorporation of dUMP residues. After a mutation occurs, the mutagenic threat of uracil propagates through any subsequent DNA replication steps. Recombinant E.coli ung protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.
Source
E. coli
Tag
His
Form
Liquid. 20mM Tris-HCl buffer (pH8.0) containing 10% glycerol
Molecular Mass
28.1 kDa(252aa) confirmed by MALDI-TOF
AA Sequence
MGSSHHHHHH SSGLVPRGSH MGSMANELTW HDVLAEEKQQ PYFLNTLQTV ASERQSGVTI YPPQKDVFNA FRFTELGDVK VVILGQDPYH GPGQAHGLAF SVRPGIAIPP SLLNMYKELE NTIPGFTRPN HGYLESWARQ GVLLLNTVLT VRAGQAHSHA SLGWETFTDK VISLINQHRE GVVFLLWGSH AQKKGAIIDK QRHHVLKAPH PSPLSAHRGF FGCNHFVLAN QWLEQRGETP IDWMPVLPAE SE
Purity
>90% by SDS - PAGE
Storage
Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
Concentration
0.5 mg/ml (determined by Bradford assay)
Official Symbol
ung
Synonyms
ung; ECK2578; JW2564; uracil-DNA-glycosylase; NP_417075.1; EC 3.2.2.-
Gene ID
Protein Refseq
