Cat.No.
RnhA-739E
Species
E. coli
Product Name
Recombinant E.coli rnhA
Product Overview
Recombinant E.coli RNase H (RNaseHI) was over-expressed inE. coliand highly purified. MW is 17.6 kDa. MW = 17.6 kDa.
Description
Ribonuclease H (RNase H) is an endoribonuclease which specifically degrades the RNA strand of an RNA/DNA hybrid, leaving the DNA strand and unhybridized RNA intact.
Source
E. coli
Form
50 units/ul in 20mM Tris-HCl (pH 7.5), 100mM KCl, 1mM DTT, 50% Glycerol.
Purity
Greater than 95% protein determined by SDS-PAGE.
Applications
1) Removal of mRNA in DNA/RNA hybrid prior to the synthesis of the second strand of cDNA .2) Removal of poly (A) tails from mRNA after hybridization with oligo (dT). 3) Oligodeoxyribonucleotide-directed site-specific cleavage of RNA.
Storage
at -20℃.
Gene Name
Synonyms
rnhA; ribonuclease HI, degrades RNA of DNA-RNA hybrids; cer; dasF; ECK0214; herA; JW0204; rnh; sdrA; sin; EC 3.1.26.4; RNase HI, degrades RNA of DNA-RNA hybrids, participates in DNA replication
Gene ID
Protein Refseq
Figure 1
productimages/rnha-739e,rnha.png
Figure Title 1
Crystallographic structure of E. coli RNase HI.
