Cat.No.
rnhA-1012E
Species
E. coli
Product Name
Recombinant E.coli RnhA, His-tagged
Product Overview
Recombinant e.colirnhA protein, fused to His-tag at N-terminus, was expressed in E.coliand purified by using conventional chromatography techniques.
Description
rnhA is an endonuclease that specifically degrades the RNA of RNA-DNA hybrids. Localized to the nucleus, this protein mediates the removal of Okazaki fragment RNA primers that are present on the lagging strand during DNA replication. rnhA catalyzes the endonucleolytic cleavage of RNA to a 5"-phosphomonoester and is able to bind magnesium or manganese as cofactors.
Form
Liquid. In 20mM Tris-HClbuffer(pH 8.0) containing 10% glycerol, 2mM DTT.
Purity
> 95 % by SDS - PAGE
Storage
Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
Concentration
1 mg/ml (determined by Bradford assay)
Gene Name
Official Symbol
Synonyms
rnhA; ribonuclease HI, degrades RNA of DNA-RNA hybrids; cer; dasF; ECK0214; herA; JW0204; rnh; sdrA; sin; EC 3.1.26.4; RNase HI, degrades RNA of DNA-RNA hybrids, participates in DNA replication
Gene ID
Protein Refseq
Figure 1
productimages/rnha-1012e rnha, his-tagged(e.coli).png
