Cat.No.
pykF-15843E
Species
E. coli
Product Name
Recombinant E.coli pykF, His-tagged
Product Overview
Recombinant E.coli pykF protein, fused to His tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
Description
pyrF (pyruvate kinase) belongs to the pyruvate kinase family. pykF is an enzyme involved in glycolysis. It catalyzes the transfer of a phosphate group from phosphoenolpyruvate (PEP) to ADP, yielding one molecule of pyruvate and one molecule of ATP. This process also requires a Magnesium ion. This step is the final one in the glycolytic pathway, which produces pyruvate molecules, the final product of aerobic glycolysis.
Source
E. coli
Tag
His
Form
Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 10% glycerol
Molecular Mass
53.3kDa (494aa)
Protein length
1-470aa
AA Sequence
MGSSHHHHHH SSGLVPRGSH MGSHMKKTKI VCTIGPKTES EEMLAKMLDA GMNVMRLNFS HGDYAEHGQR IQNLRNVMSK TGKTAAILLD TKGPEIRTMK LEGGNDVSLK AGQTFTFTTD KSVIGNSEMV AVTYEGFTTD LSVGNTVLVD DGLIGMEVTA IEGNKVICKV LNNGDLGENKGVNLPGVSIA LPALAEKDKQ DLIFGCEQGV DFVAASFIRK RSDVIEIREH LKAHGGENIH IISKIENQEG LNNFDEILEA SDGIMVARGD LGVEIPVEEV IFAQKMMIEK CIRARKVVIT ATQMLDSMIK NPRPTRAEAG DVANAILDGT DAVMLSGESA KGKYPLEAVS IMATICERTDRVMNSRLEFN NDNRKLRITE AVCRGAVETA EKLDAPLIVV ATQGGKSARA VRKYFPDATI LALTTNEKTA HQLVLSKGVV PQLVKEITST DDFYRLGKEL ALQSGLAHKG DVVVMVSGAL VPSGTTNTAS VHVL
Purity
>95% by SDS - PAGE
Applications
SDS-PAGE
Storage
Can be stored at 4°C short term. For long term storage, aliquot and store at at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
Concentration
1mg/ml (determined by Bradford assay)
Official Symbol
pykF
Synonyms
pykF; ECK1672; JW1666; pyruvate kinase I; pyruvate kinase I (formerly F), fructose stimulated
Gene ID
Protein Refseq
