Cat.No.
Skp-2573E
Species
E. coli
Product Name
Recombinant E.coli Periplasmic Chaperone, His-tagged
Product Overview
Recombinant E.coli Skp, fused to His-tag at N-terminus, was expressed inE.coliand purified by using conventional chromatography techniques. MW = 17.9 kDa (162aa).
Description
The Seventeen Kilodalton Protein (Skp) is a trimeric periplasmic chaperone that assists outer membrane proteins in their folding and insertion into membranes. This protein required for the efficient release of ompA from the inner membrane, the maintenance of its solubility in the periplasm, and, in association with lipopolysaccharide (LPS), for the efficient folding and insertion of ompA into the outer membrane.
Source
E. coli
Form
Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 20% glycerol.
Purity
> 95% by SDS – PAGE.
Storage
Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
Concentration
1mg/ml (determined by Bradford assay).
Synonyms
Skp; periplasmic chaperone; ECK0177; hlpA; JW0173; oomph; periplasmic molecular chaperone for outer membrane proteins
Gene ID
Protein Refseq