Cat.No.
nth-989E
Species
E. coli
Product Name
Recombinant E.coli Nth, His-tagged
Product Overview
Recombinant E.coli nth protein, fused to His-tag at N-terminus, was expressed in E.coliand purified by using conventional chromatography (1-211aa).
Description
Nth, also known as Endonuclease III, has an apurinic and/or apyrimidinic endonuclease activity and a DNA N-glycosylase activity. This enzyme incises damaged DNA at cytosines, thymines and guanines. Nth acts on a damaged strand, 5" from the damaged site. This enzyme is required for the repair of both oxidative DNA damage and spontaneous mutagenic lesions.
Form
Liquid. 20mM Tris-HCl buffer (pH8.0) containing 40% glycerol, 0.1M NaCl, 1mM DTT, 0.1mM PMSF
Purity
> 85% by SDS - PAGE
Storage
Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C.Avoid repeated freezing and thawing cycles.
Concentration
0.5 mg/ml (determined by Bradford assay)
Synonyms
nth; ECK1629; JW1625; DNA glycosylase and apyrimidinic (AP) lyase (endonuclease III); NP_416150.1; EC 4.2.99.18;endonuclease III; specific for apurinic and/or apyrimidinic sites
Gene ID
Protein Refseq
