Cat.No.
nfsB-2767E
Species
E. coli
Product Name
Recombinant E.coli NfsB, His-tagged
Product Overview
Recombinant E.coli NFNB protein, fused to His-tag at N-terminus, was expressed inE.coliand purified by using conventional chromatography techniques. MW = 26.0 kDa (237aa).
Description
NFNB, also known as NFSB, shows the ability to reduce quinines. This protein is an enzyme for activating prodrugs in antibody directed enzyme prodrug therapy. It also capable of reducing nitrofurazone, quinones and the anti-tumor agent CB1954 (5-(aziridin-1-yl)-2,4-dinitrobenzamide). The reduction of CB1954 results in the generation of cytotoxic species.
Source
E. coli
Form
Liquid. In 20 mM Tris-HCl buffer (pH8.0) containing 50mM Nacl, 1mM DTT, 10% glycerol.
Purity
> 95% by SDS - PAGE.
Storage
Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
Concentration
1 mg/ml (determined by Bradford assay).
Synonyms
dihydropteridine reductase, NAD(P)H-dependent, oxygen-insensitive; dprA; ECK0570; JW0567; nfnB; nfsI; ntr; oxygen-insensitive NAD(P)H nitroreductase
Gene ID
Protein Refseq
