Cat.No.
nei-53E
Species
E. coli
Product Name
Recombinant E.coli nei
Product Overview
An E. coli strain which carries the cloned Endonuclease VIII gene.
Description
E.coli Endonuclease VIII functions as both an N-glycosylase (by excising oxidative base lesions) and an AP lyase (by subsequently cleaving the phosphodiester backbone), leaving terminal phosphates at the 5′ and 3′ ends. Damaged bases removed by Endonuclease VIII include: urea, 5, 6- dihydroxythymine, thymine glycol, 5-hydroxy-5- methylhydanton, uracil glycol, 6-hydroxy-5, 6-dihydrothymine and methyltartronylurea.
Source
E. coli
Bio-activity
770,000 U/mg
Molecular Mass
29,845 Daltons
Purity
>99% by SDS-PAGE
Unit Definition
One unit is defined as the amount of enzyme required to cleave 1 pmol of an oligonucleotide duplex containing a single AP site in 1 hour at 37°C.
Storage
-25 to -15°C
Concentration
10,000 U/mL
Storage Buffer
Supplied in: 10mM Tris-HCL,250 mM NaCl, 0.1mM EDTA, 50% glycerol pH 8.0 @ 25°C. Supplied with: 10X Endonuclease Vlll Buffer: 100mM Tris-HCl, 750mM NaCl, 10mM EDTA pH 8.0 @ 25°C.
Official Symbol
nei
Synonyms
nei; endonuclease VIII; YP_488994.1; 5-formyluracil/5-hydroxymethyluracil DNA glycosylase~ECK0703:JW0704:b0714
Gene ID
Protein Refseq
UniProt ID
