Cat.No.
nanA-983E
Species
E. coli
Product Name
Recombinant E.coli NanA, His-tagged
Product Overview
Recombinant E.colinanA protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography(1-297aa).
Description
NanA, also known as N-acetylneuraminatelyase, belongs to the family of lyases, specifically the oxo-acid-lyases, which cleave carbon-carbon bonds. NanA catalyzes the cleavage of N-acetylneuraminic acid (sialic acid) to form pyruvate and N-acetyl-D-mannosamine. This protein was inhibited by reduction with NaBH4 in the presence of the substrate, indicating that it belongs to the Schiff-base-forming Class Ialdolases. NanA was strongly inhibited by Cu2+ ions, p-chloromercuribenzoate and N-bromosuccinimide, and also inhibited competitively by the reaction product, pyruvate, and its structurally related compounds, dihydroxyacetone and DL-glyceraldehyde.
Form
Liquid. 20mM Tris-HCl buffer (pH8.0) containing 20% glycerol
Purity
> 95% by SDS - PAGE
Storage
Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
Concentration
1 mg/ml (determined by Bradford assay)
Synonyms
nanA, ECK3214; JW3194; npl; N-acetylneuraminatelyase; NP_417692.1; EC 4.1.3.3; N-acetylneuraminatelyase (aldolase); catabolism of sialic acid; not K-12?
Gene ID
947742
Protein Refseq
