Cat.No.
metC-92E
Species
E. coli
Product Name
Recombinant E.coli metC, His-tagged
Product Overview
Recombinant E. coli Cystathionine Beta-Lyase MetC/metC is produced with our E. coli expression system. The target protein is expressed with sequence (Met1-Val395) of E. coli metC fused with a 6His tag at the N-terminus.
Description
Cystathionine Beta-Lyase MetC (CBL) belongs to the family of Lyases, specifically the class of carbon-sulfur lyases. CBL catalyzes the chemical reaction converting L-cystathionine to L-homocysteine, NH3 and pyruvate. This reaction depends on pyridoxal phosphate as a cofactor. CBL participates in 5 metabolic pathways: methionine metabolism, cysteine metabolism, selenoamino acid metabolism, nitrogen metabolism, and sulfur metabolism.
Source
E. coli
Tag
His
Form
Supplied as a 0.2 μM filtered solution of PBS, pH 7.4
AA Sequence
MGSSHHHHHHSSGLVPRGSHMADKKLDTQLVNAGRSKKYTLGAVNSVIQRASSLVFDSVEAKKHA TRNRANGELFYGRRGTLTHFSLQQAMCELEGGAGCVLFPCGAAAVANSILAFIEQGDHVLMTNTA YEPSQDFCSKILSKLGVTTSWFDPLIGADIVKHLQPNTKIVFLESPGSITMEVHDVPAIVAAVRS VVPDAIIMIDNTWAAGVLFKALDFGIDVSIQAATKYLVGHSDAMIGTAVCNARCWEQLRENAYLM GQMVDADTAYITSRGLRTLGVRLRQHHESSLKVAEWLAEHPQVARVNHPALPGSKGHEFWKRDFT GSSGLFSFVLKKKLNNEELANYLDNFSLFSMAYSWGGYESLILANQPEHIAAIRPQGEIDFSGTL IRLHIGLEDVDDLIADLDAGFARIV
Endotoxin
Less than 0.1 ng/µg (1 IEU/µg).
Purity
Greater than 95% as determined by SEC-HPLC and reducing SDS-PAGE.
Storage
Store at < -20°C, stable for 6 months after receipt.Please minimize freeze-thaw cycles.
