Cat.No.
IdhA-391E
Species
E. coli
Product Name
Recombinant E.coli IdhA Protein, His-tagged
Product Overview
Recombinant E.coli IdhA protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
Description
Idha, also known as D-lactate dehydrogenase, belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family. In enzymology, a D-lactate dehydrogenase (cytochrome) is an enzyme that catalyzes the chemical reaction. Thus, the two substrates of this enzyme are (D)-lactate and ferricytochrome c, whereas its two products are pyruvate and ferrocytochrome c.
Source
E. coli
Tag
His
Form
Liquid. In 20 mM Tris-HCl buffer, pH8.0, 10% glycerol, 100mM NaCl
Bio-activity
Specific activity is >200 units/mg, in which one unit will convert 1.0 umole of pyruvate to L-lactate and beta-NAD per minute at pH 7.5 at 37C.
Molecular Mass
39.1kDa (353aa), confirmed by MALDI-TOF
AA Sequence
MGSSHHHHHH SSGLVPRGSH MATLKEKLIA PVAEEEATVP NNKITVVGVG QVGMACAISI LGKSLADELA LVDVLEDKLK GEMMDLQHGS LFLQTPKIVA DKDYSVTANS KIVVVTAGVR QQEGESRLNL VQRNVNVFKF IIPQIVKYSP DCIIIVVSNP VDILTYVTWK LSGLPKHRVI GSGCNLDSAR FRYLMAEKLG IHPSSCHGWI LGEHGDSSVA VWSGVNVAGV SLQELNPEMG TDNDSENWKE VHKMVVESAY EVIKLKGYTN WAIGLSVADL IESMLKNLSR IHPVSTMVKG MYGIENEVFL SLPCILNARG LTSVINQKLK DDEVAQLKKS ADTLWDIQKD LKDL
Purity
>95% by SDS - PAGE
Storage
Can be stored at +4 centigrade short term (1-2 weeks). For long term storage, aliquot and store at -20 centigrade or -70 centigrade. Avoid repeated freezing and thawing cycles.
Concentration
1 mg/ml (determined by Bradford assay)
Official Symbol
Synonyms
ECK1377; hslF; hslI; htpH; JW1375; fermentative D-lactate dehydrogenase, NAD-dependent
Gene ID
Protein Refseq
UniProt ID
