Cat.No.
grpE-4706E
Species
E. coli
Product Name
Recombinant E.Coli Heat Shock Protein
Product Overview
GrpE Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 197 amino acids and having a molecular mass of 21.8 kDa. The GrpE is purified by proprietary chromatographic techniques.
Description
GrpE, co-chaperone of E.coli, participates actively in the response to hyperosomotic and heat shock by preventing the aggregation of stress-denatured proteins in association with DnaK. This protein is the nucleotide exchange factor for DnaK and may function as a thermosensor. Several rounds of ATP-dependent interactions between DnaJ, DnaK and GrpE are required for fully efficient folding.
Source
E. Coli
Form
The HSP24 protein solution contains 20mM Tris-HCl pH-8 and 0.1M NaCl.
Purity
Greater than 90.0% as determined by(a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
Storage
GrpE although stable 4°C for 4 weeks, should be stored desiccated below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Official Symbol
Synonyms
grpE; heat shock protein; HSP24; HSPB25.3; HSP-70 Cofactor; Protein grpE; Heat shock protein B25.3; b2614; JW2594; ECK2610
Gene ID
Protein Refseq
UniProt ID
