Recombinant E.coli GrpE Heat Shock Protein

Cat.No.

GrpE-324E

Species

E. coli

Product Name

Recombinant E.coli GrpE Heat Shock Protein

Product Overview

Recombinant GrpE protein was overexpressed in E.coli and purified by using the conventional column chromatography techniques (197 aa, 21.8 kDa).

Description

GrpE, co-chaperone of E.coli, participates actively in the response to hyperosomotic and heat shock by preventing the aggregation of stress-denatured proteins in association with DnaK. This protein is the nucleotide exchange factor for DnaK and may function as a thermosensor. Several rounds of ATP-dependent interactions between DnaJ, Dna K and GrpE are required for fully efficient folding.

Source

E. coli

Form

Liquid. In 20 mM Tris buffer (pH 8.0) containing 100 mM NaCl.

Purity

> 90% by SDS – PAGE.

Storage

Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.

Concentration

1 mg/ml (determined by Bradford assay).

Gene Name

grpE