Cat.No.
glpK-42E
Species
E. coli
Product Name
Recombinant E.coli glpK, His-tagged
Product Overview
Recombinant E.coli glpK, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
Description
glpK also known as glycerol kinase, belongs to the FGGY kinase family. glpK function to catalyze the transfer of a phosphate group from ATP to glycerol, thereby forming glycerol phosphate. This intermediate can then be converted to dihydroxyacetone phosphate (DHAP), which is utilized in either glycolysis or gluconeogenesis.
Source
E. coli
Tag
His
Form
Liquid, In Phosphate buffered saline (pH7.4) containing 10% glycerol, 1mM DTT
Molecular Mass
58.6kDa (525aa)
AA Sequence
MGSSHHHHHH SSGLVPRGSH MGSMTEKKYI VALDQGTTSS RAVVMDHDAN IISVSQREFE QIYPKPGWVE HDPMEIWATQ SSTLVEVLAK ADISSDQIAA IGITNQRETT IVWEKETGKP IYNAIVWQCR RTAEICEHLK RDGLEDYIRS NTGLVIDPYF SGTKVKWILD HVEGSRERAR RGELLFGTVD TWLIWKMTQG RVHVTDYTNA SRTMLFNIHT LDWDDKMLEV LDIPREMLPE VRRSSEVYGQ TNIGGKGGTR IPISGIAGDQ QAALFGQLCV KEGMAKNTYG TGCFMLMNTG EKAVKSENGL LTTIACGPTG EVNYALEGAV FMAGASIQWL RDEMKLINDA YDSEYFATKV QNTNGVYVVP AFTGLGAPYW DPYARGAIFG LTRGVNANHI IRATLESIAY QTRDVLEAMQ ADSGIRLHAL RVDGGAVANN FLMQFQSDIL GTRVERPEVR EVTALGAAYL AGLAVGFWQN LDELQEKAVI EREFRPGIET TERNYRYAGW KKAVKRAMAW EEHDE
Purity
>95% by SDS - PAGE
Storage
Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
Concentration
1mg/ml (determined by Bradford assay)
