Cat.No.
Exonuclease I-54E
Species
E. coli
Product Name
Recombinant E.coli Exonuclease I
Product Overview
Purified from a strain of?E. coli?that expresses the recombinant Exonuclease I gene.
Description
Exonuclease I cleaves single-stranded DNA in the 3′→5′ direction, releasing 5′ -mono/di-nucleotides and leaving double-stranded DNA molecules and the 5′-terminus intact. The enzyme is processive though digestion is inhibited by the presence of a 3′ -terminal phosphate. Exonuclease I is tolerant of a wide-range of buffer conditions and can typically be added to reactions containing magnesium.
Source
E. coli
Bio-activity
185,000 U/mg
Molecular Mass
54,500 Daltons
Purity
>99% by SDS-PAGE
Unit Definition
One unit is defined as the amount of enzyme required to produce 10 nmol of acid-soluble total nucleotide in 30 minutes at 37°C.
Storage
-15 to -25°C
Concentration
20,000 U/ml
Storage Buffer
Supplied in: 10mM Tris-HCl, 100mM NaCl, 1mM DTT, 0.5mM EDTA, 50% glycerol pH 7.5 @ 25°C.
