Cat.No.
DnaK-316E
Species
E. coli
Product Name
Recombinant E.coli DnaK Chaperone Hsp70, Co-chaperone with DnaJ (508-638)
Product Overview
Recombinant DNAK was overexpressed in E. coli and purified to apparent homogeneity by using conventional column chromatography techniques (132 aa, 14.6 kDa). Additional amino acid(Met) is attached at N- terminus.
Description
DnaK, originally identified for its DNA replication by bacteriophage l in E. coli is the bacterial hsp70 chaperone. This protein is involved in the folding and assembly of newly synthesized polypeptide chains and in preventing the aggregation of stress-denatured proteins. Dnak(residues 508-638) of the substrate binding domain is a-helical and appears to act as a lid covering the substrate binding cleft.
Source
E. coli
Form
Liquid. In 25 mM Tris-HCl buffer (pH 7.5) containing 100 mM NaCl, 1 mM DTT, 10% glycerol.
Purity
> 95% by SDS – PAGE.
Storage
Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
Concentration
1 mg/ml (determined by Bradford assay).
Gene Name
Synonyms
dnaK; dnaK chaperone Hsp70, co-chaperone with DnaJ; Hsp70 protein; ECK0014; groPAB; groPC; groPF; grpC; grpF; JW0013; seg; chaperone Hsp70; DNA biosynthesis; autoregulated heat shock proteins.
Gene ID
Protein Refseq
UniProt ID
P0A6Y8
