Cat.No.
DnaK-312E
Species
E. coli
Product Name
Recombinant E.coli DnaK Chaperone Hsp70, Co-chaperone with DnaJ (385-546)
Product Overview
Recombinant Dna K protein was overexpressed in E.coli and purified to apparent homogeneity by using conventional column chromatography techniques (163 aa, 17.7 kDa).
Description
DNAK, originally identified for its DNA replication by bacteriophage l in E. coli is the bacterial hsp70 chaperone. The protein coding region of the substrate binding domain of DNAK (amino acids 385-546) was amplified by PCR and cloned into an E. coli expression vector. The substrate binding domain of DNAK was overexpressed in E. coli and the recombinant protein was purified to apparent homogeneity by using conventional column chromatography techniques. Additional amino acid(Met) is attached at N- terminus.
Source
E. coli
Form
Liquid. In 25 mM Tris-HCl buffer (pH 7.5) containing 1 mM EDTA, 2 mM b-mercaptoethanol.
Purity
> 95% by SDS – PAGE.
Storage
Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
Concentration
1 mg/ml (determined by Bradford assay).
Gene Name
Synonyms
dnaK; dnaK chaperone Hsp70, co-chaperone with DnaJ; Hsp70 protein; ECK0014; groPAB; groPC; groPF; grpC; grpF; JW0013; seg; chaperone Hsp70; DNA biosynthesis; autoregulated heat shock proteins.
Gene ID
Protein Refseq
UniProt ID
P0A6Y8
