Cat.No.
DnaJ-317E
Species
E. coli
Product Name
Recombinant E.coli DnaJ Chaperone Hsp40, Co-chaperone with DnaK (1-376)
Product Overview
Recombinant DnaJ was overexpressed in E. coli and purified to apparent homogeneity by using conventional column chromatography techniques (376 aa, 41.1 kDa).
Description
DnaJ, Heat shock protein, functions in association with DnaK(Hsp70) molecular chaperone to facilitate protein folding. p70 chaperone. DnaJ plays a key role in the chaperone reaction by stimulating the ATPase activity and activating the substrate binding of Hsp70.. DnaJ consists of four domains that are N-terminal 76 amino acid J-domain, G/F domain, zinc-binding cystein rich CR-domain, C-terminal CTD-domain and they are conserved to various degrees among the homologues.
Source
E. coli
Form
Liquid. In 25 mM Tris-HCl buffer (pH 7.5) containing 5 mM DTT, 100 mM NaCl, 10% glycerol.
Purity
> 95% by SDS – PAGE.
Storage
Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
Concentration
1 mg/ml (determined by Bradford assay).
Gene Name
Synonyms
dnaJ; dnaJ chaperone Hsp40, co-chaperone with DnaK; ECK0015; groP; grpC; JW0014; chaperone with DnaK; heat shock protein; Hsp40 (heat shock protein 40 kD).
Gene ID
Protein Refseq
UniProt ID
P08622
