Cat.No.
DsbA-80E
Species
E. coli
Product Name
Recombinant E.coli Disulfide Oxidoreductase A
Product Overview
Recombinant DsbA (residues 20-208) was expressed in E. coli and purified by using conventional chromatography techniques, 21.1kDa (189aa).
Description
Recombinant Disulfide Oxidoreductase (rDsbA), produced from E.coli is a periplasmic protein and thioredoxin superfamily member whichintroduces disulfide bonds directly into substrate proteins by donating the disulfide bond in its active-site Cys30-Pro31-His32-Cys33 to a pair of cysteines in substrate proteins. DsbA consists of 208 amino acids containing signal peptide (1-19 amino acids).
Form
Liquid. In 20 mM Hepesl buffer (pH 7.5) containing 2 mM EDTA
Purity
> 95% by SDS - PAGE
Storage
Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
Concentration
1 mg/ml (determined by Bradford assay)
Gene Name
Synonyms
dsbA; Disulfide Oxidoreductase A; dsf; ECK3852; iarA; JW3832; ppfA; periplasmic protein disulfide isomerase I; Thiol:disulfide interchange protein dsbA
Gene ID
Protein Refseq
UniProt ID
