Cat.No.
ansA-698E
Species
E. coli
Product Name
Recombinant E.coli cytoplasmic L-asparaginase I, His-tagged
Product Overview
ansA, 1-338aa, E.coli, His tag, E.coli
Description
AnsA is the cytoplasmic asparaginase from Escherichia coli involved in intracellular asparagine utilization. Escherichia coli possesse two l-asparaginases: the cytoplasmic type I form (ansA) and the periplasmic type II form (ansB). AnsA (Type I asparaginase) is constitutively expressed and required for the growth of the bacteria on asparagine as the sole nitrogen source. Recombinant E.coli ansA protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
Source
E. coli
Tag
His
Form
Liquid. In 20mM Tris-HCl buffer(pH 8.0) containing 10% glycerol, 2mM DTT.
Molecular Mass
39.3 kDa (358aa), confirmed by MALDI-TOF
AA Sequence
MGSSHHHHHH SSGLVPRGSH MQKKSIYVAY TGGTIGMQRS EQGYIPVSGH LQRQLALMPE FHRPEMPDFT IHEYTPLMDS SDMTPEDWQH IAEDIKAHYD DYDGFVILHG TDTMAYTASA LSFMLENLGK PVIVTGSQIP LAELRSDGQI NLLNALYVAA NYPINEVTLF FNNRLYRGNR TTKAHADGFD AFASPNLPPL LEAGIHIRRL NTPPAPHGEG ELIVHPITPQ PIGVVTIYPG ISADVVRNFL RQPVKALILR SYGVGNAPQN KAFLQELQEA SDRGIVVVNL TQCMSGKVNM GGYATGNALA HAGVIGGADM TVEATLTKLH YLLSQELDTE TIRKAMSQNL RGELTPDD
Purity
>95% by SDS - PAGE
Storage
Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
Concentration
0.5mg/ml (determined by Bradford assay)
Official Symbol
ansA
Synonyms
ansA; cytoplasmic L-asparaginase I; YP_490028.1; ECK1765:JW1756:b1767
Gene ID
Protein Refseq
UniProt ID
