Cat.No.
GroEL-530E
Species
E. coli
Product Name
Recombinant E.coli Chaperonin GroEL
Product Overview
Recombinant E.coli Chaperonin GroEL was overexpressed inE.coliand was purified by using conventional chromatography techniques. MW = 57.3kDa (548aa).
Description
GroEL protein is the major heat shock protein of E.coli and belongs to the chaperonin (HSP60) family. GroEL protein prevents misfolding of proteins and promotes the refolding and proper assembly of unfolded polypeptiedes generated under stress condition. GroEL gene was amplified by PCR from E.coli and cloned into an expression vector.
Source
E. coli
Form
Liquid. In 25 mM Tris buffer (pH 7.5) containing 100 mM NaCl, 5 mM DTT, 10% glycerol.
Purity
> 95% by SDS – PAGE.
Storage
Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
Concentration
1 mg/ml (determined by Bradford assay).
Synonyms
GroEL; Chaperonin GroEL; chaperonin; heat shock protein
Gene ID
mRNA Refseq
UniProt ID
