Cat.No.
can-395E
Species
E. coli
Product Name
Recombinant E.coli can Protein, His-tagged
Product Overview
Recombinant carbonic anhydrase fused to His-tag, was expressed in E.coli and purified by conventional chromatography techniques.
Description
Carbonic anhydrase (CA) is an enzyme that catalyses rapid conversion of carbon dioxide to bicarbonate and protons (CO2 + H2O<->HCO3- + H+). Most carbonic anhydrases contain a zinc ion in their active site and the primary function of this enzyme is known to maintain acid-base balance in blood and other tissues, and to help transport carbon dioxide of tissues. Carbonic anhydrases have been found in all kingdoms of life.
Source
E. coli
Tag
His
Form
Liquid. In 20 mM Tris-HCl buffer (pH 8.0) containing 1 mM DTT, 10% glycerol
Bio-activity
Specific activity is >1,000 pmol/min/ug, and is defined as the amount of enzyme that hydrolyze 1.0 pmole of 4-nitrophenyl acetate to 4-nitrophenol per minute at pH 7.5 at 37C.
Molecular Mass
27kDa (240aa), confirmed by MALDI-TOF
AA Sequence
MGSSHHHHHH SSGLVPRGSH MKDIDTLISN NALWSKMLVE EDPGFFEKLA QAQKPRFLWI GCSDSRVPAE RLTGLEPGEL FVHRNVANLV IHTDLNCLSV VQYAVDVLEV EHIIICGHYG CGGVQAAVEN PELGLINNWL LHIRDIWFKH SSLLGEMPQE RRLDTLCELN VMEQVYNLGH STIMQSAWKR GQKVTIHGWA YGIHDGLLRD LDVTATNRET LEQRYRHGIS NLKLKHANHK
Purity
>95% by SDS - PAGE
Storage
Can be stored at +4 centigrade short term (1-2 weeks). For long term storage, aliquot and store at -20 centigrade or -70 centigrade. Avoid repeated freezing and thawing cycles.
Concentration
1 mg/ml (determined by Bradford assay)
Official Symbol
Synonyms
ECK0125; JW0122; yadF; carbonic anhydrase; putative carbonic anhdrase
Gene ID
Protein Refseq
UniProt ID
