Cat.No.
birA-339E
Species
E. coli
Product Name
Recombinant E.coli birA, His-MBP tagged
Product Overview
Recombinant E.coli birA (P06709) (Met1-Lys321), fused with an N-terminal polyhistidine-tagged MBP tag, was produced in E. coli
Source
E. coli
Tag
His/MBP
Predicted N Terminal
Met
Form
Lyophilized from sterile 50mM Tris, 100mM NaCl, 10% Glycerol, pH 8.0. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization.
Molecular Mass
The recombinant E.coil BirA/MBP fusion protein consists of 713 amino acids and has a calculated molecular mass of 78.3 kDa. It migrates as an approximately 64-68 kDa band in SDS-PAGE under reducing conditions.
Endotoxin
< 1.0 eu per μg of the protein as determined by the LAL method.
Purity
>95 % as determined by SDS-PAGE
Stability
Samples are stable for up to twelve months from date of receipt at -70oC.
Storage
Store it under sterile conditions at -20oC~-70oC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution
It is recommended that sterile water be added to the vial to prepare a stock solution. Centrifuge the vial at 4oC before opening to recover the entire contents.
